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University of Minnesota
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Methods for Experiment 271 -

aepe271 - Sapling Census

FAB Sapling Census Protocol

FAB Sapling Census Protocol

Height

Use a folding ruler to measure the height of each tree.
Measure from the base of the soil to the tip of the sapling?s leading stem.
Measure from the soil surface, not from the mulch surface. Insert ruler into mulch if necessary.
The leader is the tallest live, vegetative stem.
Measure to the base of the leader?s apical bud. Do not include bud scales or, for conifers, new needles.
If there are multiple stems, pick the tallest living stem.
Make sure that the ruler follows the contours of the stem, especially for curved stems.
Report height in centimeters to the nearest 0.5 cm. (All heights should end in ?.0? or ?.5?)

Diameter
Use calipers to measure the diameter of the sapling at 5.0 cm from the soil surface.
Before measuring and repeatedly throughout the day, check the caliper to make sure that it reads 0.0 cm when closed.
If the tree has already been measured, find the orange or pink paint pen mark indicating where it was measured previously. Use calipers to record the measurement at that point.
If the tree has not been measured previously or a paint pen mark is not apparent, measure 5.0 cm from the soil surface (see Height) using a ruler and mark the tree with a ring there. Measure from that point.
If the stem forks before 5 cm or is otherwise not amenable to measurement at 5 cm, mark and measure at a different height and note this on the data sheet.
If the stem forks and both resultant stems seem vigorous, measure below the fork.
Report Diameter in centimeters to the nearest 0.5 mm. (All diameters should end in ?.0? or ?.5?)"

aere271 - Initial soil pH

Initial soil pH -instrumentation

Orion 420A pH meter

Intital Soil pH protocol

Soils were collected at depths of 0-15cm, 15-30cm, and 30-60cm, sieved through a 2mm sieve, air dried, and 10g soil (plus 20ml DI water) weighed for pH analysis.

afee271 - Soil bulk density

Soil bulk density protocol

Soil cores were taken from 0-15cm, 15-30cm, and 30-60cm in randomly chosen FAB1 plots. The soil samples were dried and weighed. Dry weights were divided by the volume of the soil core (diameter=5cm).