University of Minnesota
University of Minnesota
College of Biological Sciences
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Nitrogen limitation in decomposition - Experiment 211


O horizon extracellular enzyme activity

Years Available: 2009

Principle Investigators

Hobbie, Sarah
Hobbie, Sarah
Ogdahl, Megan

Nitrogen limitation in decomposition - Experiment 211


O horizon extracellular enzyme activity

Years Available: 2009

Principle Investigators

View All Experiment and Dataset Methods

ATTRIBUTES

FieldNameDefinitionUnitCode
1Sitesite within Cedar Creek where experiment was conducted
2Repreplicate
3TreatmenttreatmentX = Control. no nutrient addition, IN = inorganic N addition. 10 g N m-2 y-1 as NH4NO3, ON = organic N addition. 10 g N m-2 y-1 as 3.33 g N m-2 y-1 each of alanine glycine and glutamate, C = carbon addition. 28.5 g C m-2 y-1 as D-glucose the same rates of C addition as in the ON treatment, INC= inorganic N carbon addition . 10 g N m-2 y-1 as NH4NO3 28.5 g C m-2 y-1 as D-glucose, LTN= long-term N addition. 10 g N m-2 y-1 as NH4NO3, NON= non-N nutrient addition. 10 g P m-2 y-1 and 12.6 g K m-2 y-1 as KH2PO4 10 g Ca m-2 y-1 as CaCl2 5 g Mg m-2 y-1 and 6.6 g S m-2 y-1 as MgSO4 0.5 g Fe m-2 y-1 as FeNaEDTA
4Harvestharvest time (number)
5Harvest Dateharvest time (MM/DD/YYYY)
6Wt used (g)dry mass of sample used for enzyme assaysgram
7PHOS(umol/g/h)substrate acid phosphatase activity (micromol/gram substrate/hour)micromolePerGramPerHour
8BGLU(umol/g/h)substrate beta-glucosidase activity (micromol/gram substrate/ hour)micromolesPerGramPerHour
9NAG(umol/g/h)substrate N-acetyl glucosaminidase activity (micromol/gram substrate/hour)micromolesPerGramPerHour
10CELLBIO(umol/g/h)substrate cellobiohydrolase activity (micromol/gram substrate/hour)micromolesPerGramPerHour
11POX(umol/g/h)substrate phenol oxidase activity (micromol/gram substrate/hour)micromolesPerGramPerHour
12PER(umol/g/h)substrate peroxidase activity (micromol/gram substrate/hour)micromolesPerGramPerHour
 

SAMPLE DATASET

SiteRepTreatmentHarvestHarvest DateWt used (g)PHOS umol/g/hBGLU umol/h/gNAG umol/h/gCELLBIO umol/h/gPOX umol/h/gPER umol/g/h
Oak S1C14/24/20090.4479659325.9652436342.0753186490.2385220510.98330099300
Oak S1IN14/24/20090.4536336342.9757527952.1663527351.3463867980.77014586801.301463592
Oak S1INC14/24/20090.4636442453.5166345891.4184733990.7862476710.3573948600.332738201
Oak S1LTN14/24/20090.4627372.6015904281.0265159940.5565117730.23148909700
Oak S1NON14/24/20090.4508210535.3082956051.9683363851.3708844050.68223365900
Oak S1ON14/24/20090.452779392.6606189610.8040680710.4021666380.23441756300
Oak S1X14/24/20090.4583041.5773955841.4076466590.4401600380.39354457600
Oak S2C14/24/20090.4621957944.0683644722.0862654360.912452290.92204228500
Oak S2IN14/24/20090.4597496263.900917693.5001080231.405005271.33964760100.311895822
Oak S2INC14/24/20090.4642946472.0908637932.6282230360.5062267990.6942158820.1586812210
Oak S2LTN14/24/20090.4703901952.9510225641.5195471770.5882571780.49640684200
Oak S2NON14/24/20090.4599537923.792759721.6364952910.8462485220.47630268600.956235124
            

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Nitrogen limitation in decomposition: O horizon extracellular enzyme activity

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